Unit 4: Food Contamination and Spoilage

Food Science & Nutrition Notes Third Semester

Introduction:

Definition of food contamination, primary sources of food contamination:

Food contamination refers to the presence of harmful substances or organisms in food that can cause illness or injury to humans or animals. Contaminants can be physical, chemical, or biological in nature, and they can enter the food chain at various stages, from production and processing to storage, distribution, and preparation.

There are many primary sources of food contamination, including:

  1. Biological sources: These can include bacteria, viruses, parasites, and other microorganisms that can contaminate food during production, processing, or handling. Examples of these include Salmonella, E. coli, Listeria, and Norovirus.

  2. Chemical sources: These can include toxins, pesticides, heavy metals, and other chemicals that can enter the food chain through environmental contamination or improper use of chemicals during food production or processing.

  3. Physical sources: These can include foreign objects such as glass, metal, or plastic that can accidentally enter the food chain during processing or packaging.

Contaminated food can cause a range of illnesses, from mild gastrointestinal discomfort to severe illness and even death. Therefore, it is essential to prevent food contamination through proper food handling, storage, and processing practices, as well as regular monitoring and testing of food products.

cross contamination and its preventive measures:

Cross-contamination is the transfer of harmful substances or organisms from one food item or surface to another, often through direct or indirect contact. This can occur during food production, processing, storage, distribution, and preparation, and it can pose a significant risk to public health.

To prevent cross-contamination, it is essential to follow proper food safety practices, including:

  1. Handwashing: Hands should be washed thoroughly and frequently with soap and warm water, especially before and after handling raw food, using the restroom, or touching surfaces that may be contaminated.

  2. Separate raw and cooked foods: Raw meat, poultry, seafood, and eggs should be stored separately from cooked or ready-to-eat foods to prevent cross-contamination. Use separate cutting boards, utensils, and dishes for raw and cooked foods.

  3. Cook food to the proper temperature: Cooking food to the appropriate temperature can kill harmful bacteria and other microorganisms that may be present. Use a food thermometer to ensure that food is cooked to the correct temperature.

  4. Proper storage: Store food at the appropriate temperature to prevent bacterial growth. Keep cold foods below 40°F and hot foods above 140°F. Refrigerate or freeze perishable food within two hours of preparation.

  5. Cleaning and sanitizing: Clean and sanitize all surfaces, utensils, and equipment that come into contact with food. Use hot, soapy water to clean surfaces and utensils, and use a sanitizing solution to kill any remaining bacteria or viruses.

Causes of food spoilage:

Food spoilage refers to the deterioration of food quality and safety due to the growth of microorganisms, enzymatic reactions, and other chemical and physical processes. There are several causes of food spoilage, including:

  1. Microbial growth: Microorganisms such as bacteria, yeast, and mold can grow on food and cause spoilage. Factors such as temperature, moisture, pH, and oxygen levels can affect the growth of these microorganisms.

  2. Enzymatic reactions: Enzymes present in food can cause spoilage by catalyzing chemical reactions that degrade food quality. For example, enzymes can cause fruits and vegetables to ripen and soften, leading to spoilage if not consumed promptly.

  3. Chemical reactions: Chemical reactions can cause food spoilage by altering the color, texture, and flavor of food. For example, fats in food can become rancid over time, causing an off-flavor and odor.

  4. Physical damage: Physical damage such as bruising, crushing, or puncturing can cause food to spoil by providing a site for microbial growth or by accelerating enzymatic and chemical reactions.

  5. Environmental factors: Environmental factors such as temperature, humidity, and light can contribute to food spoilage. For example, exposure to sunlight can cause the degradation of some vitamins and nutrients in food.

To prevent food spoilage, it is essential to store food properly, maintain proper hygiene and sanitation practices, and monitor food quality regularly. This includes keeping perishable foods refrigerated or frozen, using food before its expiration date, and properly cleaning and sanitizing equipment and surfaces that come into contact with food.

Growth and activity of microorganisms:

Microorganisms are living organisms that are too small to be seen without a microscope. They include bacteria, viruses, fungi, and parasites. Microorganisms play important roles in food production, such as fermentation and cheese-making, but they can also cause food spoilage and foodborne illness.

The growth and activity of microorganisms are affected by several factors, including:

  1. Temperature: Microorganisms have different temperature requirements for growth. For example, pathogenic bacteria such as Salmonella and E. coli grow best at warm temperatures, while spoilage bacteria and fungi can grow at lower temperatures.

  2. pH: The pH of food can affect the growth and activity of microorganisms. For example, acid-tolerant bacteria such as Lactobacillus can grow in acidic environments such as in fermented foods like yogurt.

  3. Water activity: The water activity or moisture content of food can affect microbial growth. Foods with low water activity, such as dried fruits or nuts, are less likely to support microbial growth than high-moisture foods like fresh fruits and vegetables.

  4. Oxygen: The presence or absence of oxygen can affect the growth and activity of microorganisms. For example, some bacteria require oxygen to grow, while others can grow in the absence of oxygen.

  5. Nutrients: Microorganisms require nutrients such as carbohydrates, proteins, and fats to grow and reproduce. Foods that are rich in nutrients can support microbial growth.

To control the growth and activity of microorganisms, it is essential to implement good hygiene and sanitation practices, use proper storage and handling techniques, and follow safe food processing and cooking methods. This includes maintaining proper temperatures for storage and cooking, avoiding cross-contamination, and using preservatives and other antimicrobial agents when necessary.

chemical reactions:

Chemical reactions can occur in food and affect its quality and safety. These reactions can be initiated by enzymes or by chemical compounds naturally present in the food or added during processing. Some of the chemical reactions that can occur in food include:

  1. Oxidation: Oxidation is a chemical reaction that occurs when oxygen reacts with a substance, leading to the degradation of food quality. For example, the oxidation of fats can lead to rancidity, which causes off-flavors and odors.

  2. Hydrolysis: Hydrolysis is a chemical reaction that involves the addition of water to a compound, leading to its breakdown. For example, the hydrolysis of carbohydrates can lead to the formation of simple sugars, which can impact the texture and sweetness of the food.

  3. Maillard reaction: The Maillard reaction is a chemical reaction between amino acids and reducing sugars that occurs during cooking. This reaction gives food a brown color and enhances its flavor.

  4. Enzymatic browning: Enzymatic browning occurs when enzymes in fruits and vegetables react with oxygen, leading to discoloration and degradation of quality. This can be prevented by adding acid or by blanching the fruits or vegetables.

  5. Fermentation: Fermentation is a process in which microorganisms convert carbohydrates into alcohol, acids, and gases, leading to changes in flavor, texture, and aroma.

To prevent undesirable chemical reactions in food, it is important to control the temperature, pH, and water activity of food during processing and storage. Adding antioxidants or preservatives can also help to prevent oxidation and microbial growth. Additionally, using proper storage and handling techniques, such as storing food in airtight containers, can help to preserve its quality and safety.

Natural Enzymes:

Enzymes are proteins that act as biological catalysts, speeding up chemical reactions in living organisms. Enzymes are naturally present in many foods and play important roles in food processing and digestion. Natural enzymes can be found in fruits, vegetables, grains, meat, and dairy products.

Some examples of natural enzymes in food include:

  1. Amylase: Amylase is an enzyme that breaks down carbohydrates into simple sugars. Amylase is found in foods such as grains, potatoes, and fruits.

  2. Protease: Protease is an enzyme that breaks down proteins into amino acids. Protease is found in meat, dairy products, and some fruits such as papayas and pineapples.

  3. Lipase: Lipase is an enzyme that breaks down fats into fatty acids and glycerol. Lipase is found in milk, cheese, and some fruits such as avocados.

  4. Lactase: Lactase is an enzyme that breaks down lactose, the sugar found in milk, into glucose and galactose. Lactase is naturally present in milk and dairy products.

Natural enzymes can be beneficial in food processing, as they can help to break down complex molecules into simpler ones, making the food easier to digest and improving its texture and flavor. However, natural enzymes can also lead to spoilage and degradation of quality if not properly controlled. For example, the natural enzymes in fruits and vegetables can lead to browning and degradation of texture and flavor if the produce is not properly stored and handled.

Food processors can also use commercial enzymes to improve the quality and safety of food. Commercial enzymes can be used to improve the texture of baked goods, enhance the flavor of cheese, or break down the proteins in meat to tenderize it. However, it is important to use these enzymes in accordance with food safety regulations and to properly label foods that contain them for consumers with food allergies.

Damage by animal:

Animal damage can occur during the production, storage, and transport of food. Some common forms of animal damage include:

  1. Physical damage: Animals such as rodents, birds, and insects can physically damage food by chewing, scratching, or pecking at it. This can result in contamination, loss of product, and reduced quality.

  2. Microbial contamination: Animals can carry harmful bacteria, viruses, and other microorganisms that can contaminate food and cause illness. For example, rodents can carry Salmonella and E. coli, which can cause foodborne illness in humans.

  3. Chemical contamination: Animals can also contaminate food with chemicals such as pesticides or veterinary drugs. For example, if animals are grazing in fields that have been sprayed with pesticides, the residue can accumulate in their bodies and be transferred to the food they produce.

Preventive measures to minimize animal damage in food production and storage include:

  1. Proper storage: Food should be stored in containers that are sealed and protected from pests and animals. This includes proper storage of feed and raw materials used in food production.

  2. Pest control: Regular pest control measures should be implemented to prevent infestations of rodents, birds, and insects.

  3. Sanitation: Proper sanitation practices should be followed to prevent the growth and spread of harmful microorganisms.

  4. Animal husbandry practices: Animal husbandry practices should be followed to prevent contamination of animal products with chemicals and other contaminants.

  5. Regulatory compliance: Regulations and standards related to food safety and animal welfare should be followed to ensure that animals are raised and handled in a way that minimizes the risk of food contamination.

Insects, and Rodents:

Insects and rodents are common pests that can cause damage to food during production, storage, and transport. They can contaminate food with harmful microorganisms and cause physical damage to the food product.

Insects such as beetles, moths, and flies can lay eggs on food, which can hatch into larvae that can feed on the food and cause contamination. Insects can also contaminate food with bacteria and other microorganisms that can cause foodborne illness in humans.

Rodents such as mice and rats can also cause physical damage to food by chewing and gnawing on packaging and containers. They can also contaminate food with their feces and urine, which can contain harmful bacteria and viruses.

Preventive measures to minimize the damage caused by insects and rodents include:

  1. Proper storage: Food should be stored in containers that are sealed and protected from pests. This includes proper storage of raw materials used in food production.

  2. Pest control: Regular pest control measures should be implemented to prevent infestations of insects and rodents. This can include the use of traps, baits, and insecticides.

  3. Sanitation: Proper sanitation practices should be followed to prevent the growth and spread of harmful microorganisms.

  4. Structural maintenance: Structural issues such as cracks and gaps in walls and floors should be addressed to prevent pests from entering the facility.

  5. Monitoring: Regular monitoring should be conducted to detect and address any signs of pest infestation.

  6. Regulatory compliance: Regulations and standards related to food safety and pest control should be followed to ensure that food products are safe and free from contamination.

Introduction:

Methods of food preservation:

Food preservation is the process of extending the shelf life of food by inhibiting the growth of microorganisms, delaying the onset of spoilage, and preventing chemical and physical changes that can affect the quality of the food. There are several methods of food preservation that are commonly used:

  1. Thermal processing: This method involves the application of heat to food to destroy microorganisms and enzymes that can cause spoilage. Examples of thermal processing methods include canning, pasteurization, and sterilization.

  2. Refrigeration and freezing: These methods involve the use of low temperatures to slow down the growth of microorganisms and enzymes that can cause spoilage. Refrigeration is typically used for short-term preservation, while freezing is used for long-term preservation.

  3. Drying: This method involves the removal of moisture from food, which inhibits the growth of microorganisms and enzymes that can cause spoilage. Examples of drying methods include sun-drying, oven-drying, and freeze-drying.

  4. Salting and curing: These methods involve the use of salt and other compounds to preserve food by inhibiting the growth of microorganisms and enzymes. Examples of salting and curing methods include salt-curing, smoking, and pickling.

  5. Fermentation: This method involves the use of microorganisms to convert sugars and other nutrients in food into acids and alcohol, which inhibits the growth of harmful bacteria and can enhance the flavor of the food. Examples of fermented foods include yogurt, sauerkraut, and kimchi.

  6. Chemical preservation: This method involves the use of chemical compounds such as preservatives to inhibit the growth of microorganisms and enzymes that can cause spoilage. Examples of chemical preservatives include sodium benzoate, potassium sorbate, and nitrates.

Each method of food preservation has its own advantages and disadvantages, and the choice of method depends on factors such as the type of food being preserved, the desired shelf life, and the availability of resources and equipment.

pasteurization:

Pasteurization is a thermal processing method used to kill harmful microorganisms in food by heating it to a specific temperature for a specified time. The process is named after Louis Pasteur, a French scientist who first discovered the process in the 19th century.

The most common application of pasteurization is in the dairy industry, where it is used to reduce the risk of foodborne illness by destroying harmful bacteria such as Salmonella and Listeria that may be present in milk and milk products. The process involves heating the milk to a temperature of 71.7°C (161°F) for 15 seconds, followed by rapid cooling to below 4°C (39°F) to prevent the growth of any surviving bacteria.

There are two main methods of pasteurization:

  1. High-Temperature Short-Time (HTST) pasteurization: In this method, the food is heated to a high temperature for a short time, usually 72°C (162°F) for 15 seconds. This method is commonly used in the dairy industry.

  2. Low-Temperature Long-Time (LTLT) pasteurization: In this method, the food is heated to a lower temperature for a longer time, usually 63°C (145°F) for 30 minutes. This method is commonly used for certain types of juices and other beverages.

Pasteurization is an effective method of food preservation as it can kill harmful microorganisms without significantly altering the taste, texture, or nutritional value of the food. However, it does not sterilize the food, and some microorganisms may survive the process. Therefore, proper handling and storage practices are still required to ensure the safety of pasteurized food.

canning:

Canning is a method of preserving food by sealing it in airtight containers and heating it to a high temperature to kill microorganisms and enzymes that can cause spoilage. The process was developed in the 19th century and is still widely used today.

The canning process typically involves the following steps:

  1. Preparation: The food is cleaned, cut, and cooked as necessary.

  2. Filling: The food is packed into clean, sterilized containers, usually made of glass or metal.

  3. Sealing: The containers are sealed with lids and bands to create an airtight seal.

  4. Processing: The sealed containers are heated to a high temperature to kill any microorganisms and enzymes that may be present. This process is called “processing” or “cooking.”

  5. Cooling: The processed containers are cooled and checked for airtight seals.

Canning can be done using a variety of methods, including water bath canning and pressure canning. Water bath canning is used for high-acid foods such as fruits, tomatoes, and pickles, while pressure canning is used for low-acid foods such as meats, vegetables, and soups. The choice of method depends on the type of food being canned and its acidity level.

Canned food has a long shelf life and can be stored at room temperature. However, it is important to store canned food in a cool, dry place away from light and heat to maintain its quality and safety. Canned food should also be checked for signs of spoilage such as bulging, leaking, or unusual odors before consuming.

Refrigeration:

 

Refrigeration is a method of preserving food by keeping it at a low temperature to slow down or inhibit the growth of microorganisms that can cause spoilage. It is one of the most common methods of food preservation used today.

Refrigeration works by slowing down the activity of microorganisms and enzymes that can cause food to spoil. At temperatures between 0°C (32°F) and 5°C (41°F), most bacteria and fungi are unable to grow and reproduce, which helps to keep food fresh for longer periods of time.

Refrigeration can be used for a wide range of food items, including fresh fruits and vegetables, dairy products, meat, poultry, and seafood. However, it is important to note that refrigeration only slows down the growth of microorganisms, and does not completely stop it. Therefore, perishable foods should still be consumed within a reasonable time frame to ensure their safety and quality.

To ensure that food is properly refrigerated, it is important to follow certain guidelines, such as:

  1. Store perishable food items in the refrigerator within two hours of purchase or preparation.

  2. Keep the refrigerator temperature at or below 4°C (40°F).

  3. Store raw meat, poultry, and seafood on the bottom shelf of the refrigerator to prevent cross-contamination with other foods.

  4. Keep the refrigerator clean and free of spills and food debris.

Freezing:

Freezing is a method of food preservation that involves lowering the temperature of food to below its freezing point, typically to around -18°C (0°F), in order to slow down or stop the growth of microorganisms and enzymes that can cause spoilage. Freezing is one of the most effective methods of food preservation and can extend the shelf life of many foods for several months.

Freezing works by reducing the temperature of the food to a point where most microorganisms and enzymes become inactive. This slows down the rate of chemical reactions and bacterial growth, which can help to preserve the quality and freshness of the food. Freezing can also help to retain the nutritional value of food, as well as its flavor and texture.

Freezing can be used for a wide range of food items, including fruits and vegetables, meats, poultry, seafood, breads, and prepared meals. However, it is important to note that not all foods freeze well. Some foods, such as lettuce and other leafy greens, may become wilted and mushy when frozen. Others, such as dairy products and mayonnaise-based salads, may separate or curdle when thawed.

To ensure that food is properly frozen, it is important to follow certain guidelines, such as:

  1. Freeze food as soon as possible after it is prepared or purchased.

  2. Package food in airtight containers or freezer bags to prevent freezer burn and moisture loss.

  3. Label frozen food with the date of freezing to ensure that it is used within a reasonable time frame.

  4. Thaw frozen food in the refrigerator or in a microwave oven, and avoid thawing food at room temperature.

Increasing the acidity:

Increasing the acidity of food is a method of food preservation that involves adding acidic ingredients, such as vinegar, lemon juice, or citric acid, to the food. The acidic environment helps to inhibit the growth of bacteria and other microorganisms that can cause spoilage.

Increasing the acidity of food is a common preservation method for foods such as pickles, relishes, and chutneys. These foods are typically made by immersing vegetables or fruits in a solution of vinegar or other acidic ingredients, along with various spices and seasonings. The high acidity of the solution helps to prevent the growth of bacteria and other microorganisms, and also enhances the flavor of the food.

Increasing the acidity of food can also be used to preserve fresh fruits and vegetables. For example, soaking sliced apples in lemon juice can help to prevent browning and spoilage. Similarly, adding vinegar to tomato sauce can help to preserve the sauce and enhance its flavor.

It is important to note that increasing the acidity of food can also affect its taste and texture. Foods that are too acidic may taste sour or bitter, and may also have a softer texture due to the breakdown of the food’s structure. Therefore, it is important to use acidic ingredients in moderation and to follow a recipe carefully to ensure that the desired taste and texture is achieve.

Drying:

Drying is a method of food preservation that involves removing the moisture from food in order to prevent the growth of microorganisms and enzymes that can cause spoilage. Drying can be accomplished through a variety of methods, including sun-drying, oven-drying, and dehydration using specialized equipment.

Dried foods have been used for thousands of years as a way of preserving food for long-term storage. Dried foods are lightweight, compact, and have a long shelf life, making them ideal for storage and transportation. Some commonly dried foods include fruits, vegetables, herbs, spices, and meats.

Drying works by removing the water content from food, which makes it difficult for microorganisms to survive and thrive. Without water, enzymes and bacteria are unable to carry out the chemical reactions that lead to spoilage. Drying also concentrates the flavor of the food, making it more intense and flavorful.

There are several methods of drying food, each with its own advantages and disadvantages. Sun-drying involves placing food in the sun and allowing it to dry naturally, but can be slow and unpredictable. Oven-drying involves placing food in an oven and heating it to a low temperature, but can be energy-intensive and time-consuming. Dehydration using specialized equipment, such as a dehydrator, involves circulating warm air over the food to remove moisture, and is a more efficient and consistent method of drying food.

To ensure that food is properly dried, it is important to follow certain guidelines, such as:

  1. Cut food into uniform pieces to ensure even drying.

  2. Use ripe, fresh produce to achieve the best quality dried product.

  3. Dry food in a well-ventilated area to prevent the growth of mold.

  4. Store dried food in airtight containers in a cool, dry place to prevent moisture absorption.

By following these guidelines, drying can be an effective method of preserving food and extending its shelf life, while also enhancing its flavor and nutritional value.

Using chemical preservatives:

Using chemical preservatives is a method of food preservation that involves adding synthetic or natural chemicals to food to prevent the growth of microorganisms and enzymes that can cause spoilage. Chemical preservatives work by inhibiting the growth of bacteria, mold, yeast, and other microorganisms, and by preventing or slowing down the oxidation of fats and oils in food.

There are a variety of chemical preservatives that can be used in food, including:

  1. Benzoates: This type of preservative is commonly used in acidic foods such as carbonated drinks, fruit juices, and pickles.

  2. Sorbates: Sorbates are often used in baked goods, cheese, and fruit preserves.

  3. Nitrites: Nitrites are commonly used in cured meats, such as ham and bacon, to prevent the growth of Clostridium botulinum bacteria, which can cause botulism.

  4. Sulfites: Sulfites are often used in wine, dried fruit, and processed foods to prevent the growth of bacteria and mold.

While chemical preservatives can be effective in extending the shelf life of food, there are concerns about their potential health effects. Some people may be sensitive or allergic to certain preservatives, and some studies have linked certain preservatives to health problems such as cancer and asthma.

Therefore, the use of chemical preservatives is regulated by food safety agencies in many countries, and the amounts and types of preservatives that can be used in food are strictly controlled. In addition, many consumers are increasingly seeking out food products that are free from artificial preservatives and are instead opting for natural alternatives, such as vinegar, salt, and citric acid, to preserve their food.

 

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